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ProductsBack/Annexin A1 antibody
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX113329 diluted at 1:10000
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX113329 diluted at 1:10000
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX113329 diluted at 1:10000

Annexin A1 antibody

GTX113329
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetANXA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Annexin A1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID301
  • Target name
    ANXA1
  • Target description
    annexin A1
  • Target synonyms
    ANX1, LPC1, annexin A1, annexin I (lipocortin I), annexin-1, calpactin II, calpactin-2, chromobindin-9, epididymis secretory sperm binding protein, phospholipase A2 inhibitory protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04083
  • Protein Name
    Annexin A1
  • Scientific Description
    Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Alhasan H, Terkawi MA, Matsumae G, et al. Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis. Nat Commun. 2022,13(1):3919. doi: 10.1038/s41467-022-31646-0
    Read this paper
  • Chang SJ, Liao EC, Yeo HY, et al. Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma. Arch Biochem Biophys. 2018,647:10-32. doi: 10.1016/j.abb.2018.04.004
    Read this paper
  • Gueugneau M, Coudy-Gandilhon C, Gourbeyre O, et al. Proteomics of muscle chronological ageing in post-menopausal women. BMC Genomics. 2014,15:1165. doi: 10.1186/1471-2164-15-1165
    Read this paper

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Figure 1. Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (A01451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A1/ANXA1 antigen affinity purified polyclonal antibody (Catalog # A01451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 35 kDa. The expected band size for Annexin A1/ANXA1 is at 39 kDa.
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