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ProductsBack/Annexin A4/Annexin IV Rabbit mAb

Annexin A4/Annexin IV Rabbit mAb

A9203
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetANXA4
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Overview

  • Supplier
    ABclonal
  • Product Name
    Annexin A4/Annexin IV Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID307
  • Target name
    ANXA4
  • Target description
    annexin A4
  • Target synonyms
    ANX4, HEL-S-274, P32.5, PAP-II, PIG28, PP4-X, ZAP36, annexin A4, 35-beta calcimedin, annexin IV (placental anticoagulant protein II), annexin-4, carbohydrate-binding protein p33/p41, chromobindin-4, endonexin I, epididymis secretory protein Li 274, lipocortin IV, placental anticoagulant protein II, proliferation-inducing gene 28, proliferation-inducing protein 28, protein II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09525
  • Protein Name
    Annexin A4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Western blot All lanes: Annexin A4 antibody at 4ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 28 kDa Observed band size: 36 kDa
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Figure 1. Western blot analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin-4/ANXA4 antigen affinity purified polyclonal antibody (Catalog # A04840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin-4/ANXA4 at approximately 36 kDa. The expected band size for Annexin-4/ANXA4 is at 36 kDa.
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