Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin II(GTX100046) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
![Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100046/GTX100046_40240_20170112_WB_KO_watermark_w_23053123_987.webp "Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Annexin II antibody [N1], N-term detects ANXA2 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg HCT116 whole cell lysate/extract 10% SDS-PAGE Annexin II antibody [N1], N-term (GTX100046) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100046/GTX100046_40240_WB_w_23053123_408.webp "Annexin II antibody [N1], N-term detects ANXA2 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg HCT116 whole cell lysate/extract 10% SDS-PAGE Annexin II antibody [N1], N-term (GTX100046) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin II(GTX100046) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Annexin II antibody [N1], N-term
GTX100046
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetANXA2
Overview
- SupplierGeneTex
- Product NameAnnexin II antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.75 mg/ml
- ConjugateUnconjugated
- Gene ID302
- Target nameANXA2
- Target descriptionannexin A2
- Target synonymsANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV, annexin A2, annexin II, annexin-2, calpactin I heavy chain, calpactin I heavy polypeptide, calpactin-1 heavy chain, chromobindin 8, epididymis secretory protein Li 270, epididymis secretory sperm binding protein, lipocortin II, placental anticoagulant protein IV, protein I
- HostRabbit
- IsotypeIgG
- Protein IDP07355
- Protein NameAnnexin A2
- Scientific DescriptionThis gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012Read this paper
Datasheet
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