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Western Blot analysis of HeLa cells using Annexin II Polyclonal Antibody
Western Blot analysis of HeLa cells using Annexin II Polyclonal Antibody
Western Blot analysis of HeLa cells using Annexin II Polyclonal Antibody

ANXA2 Antibody

CSB-PA000879
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetANXA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANXA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID302
  • Target name
    ANXA2
  • Target description
    annexin A2
  • Target synonyms
    ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV, annexin A2, annexin II, annexin-2, calpactin I heavy chain, calpactin I heavy polypeptide, calpactin-1 heavy chain, chromobindin 8, epididymis secretory protein Li 270, epididymis secretory sperm binding protein, lipocortin II, placental anticoagulant protein IV, protein I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07355
  • Protein Name
    Annexin A2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Annexin A2/ANXA2 using anti-Annexin A2/ANXA2 antibody (A00868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A2/ANXA2 antigen affinity purified polyclonal antibody (Catalog # A00868-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A2/ANXA2 at approximately 36 kDa. The expected band size for Annexin A2/ANXA2 is at 39 kDa.
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Lane 1: Hela Cells; Lane 2: Human Kidney; Lane 3: Mouse Testis; Probed with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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