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WB analysis of wild type and Annexin III knock out HeLa cell lysate(10microg per lane) using GTX84883 Annexin III antibody [1A9]. Dilution : 1:500
WB analysis of wild type and Annexin III knock out HeLa cell lysate(10microg per lane) using GTX84883 Annexin III antibody [1A9]. Dilution : 1:500
WB analysis of wild type and Annexin III knock out HeLa cell lysate(10microg per lane) using GTX84883 Annexin III antibody [1A9]. Dilution : 1:500

Annexin III antibody [1A9]

GTX84883
GeneTex
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetANXA3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Annexin III antibody [1A9] - KO/KD, Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1A9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID306
  • Target name
    ANXA3
  • Target description
    annexin A3
  • Target synonyms
    ANX3, annexin A3, 35-alpha calcimedin, Annexin III (lipocortin III), PAP-III, annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha), annexin-3, calcimedin 35-alpha, inositol 1,2-cyclic phosphate 2-phosphohydrolase, lipocortin III, placental anticoagulant protein III
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12429
  • Protein Name
    Annexin A3
  • Scientific Description
    Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANXA3 using anti-ANXA3 antibody (A04796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA3 antigen affinity purified polyclonal antibody (Catalog # A04796) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANXA3 at approximately 36 kDa. The expected band size for ANXA3 is at 36 kDa.
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