Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [GT1068] (GTX634219) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![ANP antibody [GT1068] detects ANP protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX634219/GTX634219_44713_20220808_ICC_IF_22081423_207.webp "ANP antibody [GT1068] detects ANP protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![ANP antibody [GT1068] detects secreted ANP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX634219/GTX634219_44713_20220909_IHC-P_M_22101319_579.webp "ANP antibody [GT1068] detects secreted ANP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Sandwich ELISA detection of recombinant Human ANP protein, GST tag (GTX109255-pro) using antibodies as below. Capture: ANP antibody [GT1068] (GTX634219) (5 μg/mL) Detection: ANP antibody [HL2617] (GTX639071) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX634219/GTX634219_44713_20231013_ELISA_PAIR_23101921_696.webp "Sandwich ELISA detection of recombinant Human ANP protein, GST tag (GTX109255-pro) using antibodies as below. Capture: ANP antibody [GT1068] (GTX634219) (5 μg/mL) Detection: ANP antibody [HL2617] (GTX639071) (1 μg/mL)")
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [GT1068] (GTX634219) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
ANP antibody [GT1068]
GTX634219
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNPPA
Overview
- SupplierGeneTex
- Product NameANP antibody [GT1068]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1068
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4878
- Target nameNPPA
- Target descriptionnatriuretic peptide A
- Target synonymsANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND, natriuretic peptides A, atrial natriuretic factor prohormone, atrial natriuretic peptide prohormone, atriopeptigen, atriopeptin, cardiodilatin-related peptide, cardionatrin, natriuretic peptide precursor A, preproANP, preproCDD-ANF, prepronatriodilatin, proANF, proANP
- HostMouse
- IsotypeIgG1
- Protein IDP01160
- Protein NameNatriuretic peptides A
- Scientific DescriptionThe protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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