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ProductsBack/Anti-4-1BB [LOB12.3]

Anti-4-1BB [LOB12.3]

AB01052-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, Other Application
Product group Antibodies
TargetTnfrsf9
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-4-1BB [LOB12.3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody can be used to detect mouse 4-1BB (CD137). In its original rat IgG1 format, this antibody had agonistic effect and prolonged survival of mice bearing CT26 or A31 tumors. In the original publication another clone LOB12 disclosed which has a rat IgG2a isotype. This has led to some confusion in the literature with regards to the original isotype of LOB12 and LOB12.3. We offer this antibody in both formats as well as murine versions so that researchers may choose the isotype most appropriate for their assays.
  • Applications
    Flow Cytometry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LOB12.3
  • Gene ID21942
  • Target name
    Tnfrsf9
  • Target description
    tumor necrosis factor receptor superfamily, member 9
  • Target synonyms
    4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; ILA; Ly6; Ly63; secreted CD137 antigen; T-cell antigen 4-1BB; tumor necrosis factor receptor superfamily member 9
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20334
  • Protein Name
    Tumor necrosis factor receptor superfamily member 9
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD137 using anti-CD137 antibody (A00867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD137 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD137 antigen affinity purified polyclonal antibody (Catalog # A00867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD137 at approximately 65KD. The expected band size for CD137 is at 44KD.
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