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CD137 antibody [3H3]

GTX14663
GeneTex
ApplicationsOther Application
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Product group Antibodies
TargetTnfrsf9
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD137 antibody [3H3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H3
  • Conjugate
    Unconjugated
  • Gene ID21942
  • Target name
    Tnfrsf9
  • Target description
    tumor necrosis factor receptor superfamily, member 9
  • Target synonyms
    4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; ILA; Ly6; Ly63; secreted CD137 antigen; T-cell antigen 4-1BB; tumor necrosis factor receptor superfamily member 9
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP20334
  • Protein Name
    Tumor necrosis factor receptor superfamily member 9
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD137 using anti-CD137 antibody (A00867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD137 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD137 antigen affinity purified polyclonal antibody (Catalog # A00867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD137 at approximately 65KD. The expected band size for CD137 is at 44KD.
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A) FACS: Mouse CD137-transfected HEK 293 cells were stained significantly using anti-CD137 (mouse), mAb (M4173) (Prod. No. AG-20A-0072). No binding was detected on empty vector transfected cells. B) FACS: Mouse T lymphocyte cells (treated Con A) were stai
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