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Immunohistochemical staining of human Liver cancer shows moderate extracellular space positivity in tumor cells.
Immunohistochemical staining of human Liver cancer shows moderate extracellular space positivity in tumor cells.
Immunohistochemical staining of human Liver cancer shows moderate extracellular space positivity in tumor cells.

Anti-A1BG Antibody

HPA044252
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetA1BG
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-A1BG
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1
  • Target name
    A1BG
  • Target description
    alpha-1-B glycoprotein
  • Target synonyms
    A1B; ABG; alpha-1B-glycoprotein; epididymis secretory sperm binding protein Li 163pA; GAB; HEL-S-163pA; HYST2477
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04217
  • Protein Name
    Alpha-1B-glycoprotein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Lane 1: MBA-MB-231 cell lysates; Lane 2: Raji cell lysates probed with A1BG Polyclonal Antibody, Unconjugated (bs-6772R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Alpha 1B-Glycoprotein/A1BG using anti-Alpha 1B-Glycoprotein/A1BG antibody (A07289-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: rat plasma lysates, Lane 4: mouse plasma lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1B-Glycoprotein/A1BG antigen affinity purified polyclonal antibody (Catalog # A07289-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1B-Glycoprotein/A1BG at approximately 50-75 kDa. The expected band size for Alpha 1B-Glycoprotein/A1BG is at 54 kDa.
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