Anti-A33 [huA33]

Absolute Antibody

Anti-A33 [huA33]

7

hA33 binds specifically to the ectodomain of A33 cell-surface differentation antigen. The antigen is a single-pass type-1 membrane glyoprotein of the immunoglobulin superfamily and, while not present in most normal and tumor cell type, it is expressed on

The binding specificity and affinity of the humanized antibody has been shown to be equal to that of the orginal mouse antibody (1.3 nM), as is the uptake of 90Y-labelled antibody by human coloractal xenografts in nude mice. The circulating half-life of huA33 is also greater than that of the mouse antibody. Antigen-binding assays have been conducted by ELISA using Colo205 and SWI222 A33 antigen-expressing cells. Biodistribution assays have also been performed using hA33-12N4 conjugate in nude mice bearing subcutaneous SWI222 tumour xenografts. This antibody can be used as a biomarker for colorectal carcinoma.TFM (trivalent Fab fragment - constructed using trimaleimide cross-linkers) was shown to have a greater binding avidity for the target compared to huA33 with faster blood clearance shown in cynomolgus monkeys and guinea pigs.

ImmunoFluorescence, ELISA, ImmunoHistoChemistry, Other Application

deplete; ELISA; IF; IHC

Research Use Only

Monoclonal

huA33

GPA33

glycoprotein A33

A33; cell surface A33 antigen; glycoprotein A33 (transmembrane); transmembrane glycoprotein A33

Human

IgG1

Cell surface A33 antigen

Human

Human

hA33 was generated by humanization of mouse A33 IgG2a-kappa by CDR-grafting, and expressed in NSO cells. The mouse A33 antibody was prepared by immunizing BALB/c mice with AsPC-1 pancreatic adenocarcinoma cell line SP2/0 myeloma cells.

-20°C,2°C to 8°C

12352203