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Immunohistochemical staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.

Anti-ABHD5 Antibody

HPA035852
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABHD5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ABHD5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51099
  • Target name
    ABHD5
  • Target description
    abhydrolase domain containing 5, lysophosphatidic acid acyltransferase
  • Target synonyms
    CGI58, IECN2, NCIE2, 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, abhydrolase domain-containing protein 5, lipid droplet-binding protein CGI-58
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WTS1
  • Protein Name
    1-acylglycerol-3-phosphate O-acyltransferase ABHD5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Abhd5 using anti-Abhd5 antibody (PB10021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: A431 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Abhd5 antigen affinity purified polyclonal antibody (Catalog # PB10021) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Abhd5 at approximately 43KD. The expected band size for Abhd5 is at 39KD.
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