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FACS: HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F) (Prod. No. AG-20A-0032).
FACS: HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F) (Prod. No. AG-20A-0032).
FACS: HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F) (Prod. No. AG-20A-0032).

anti-ACE2 (human), mAb (AC18F)

AG-20A-0032
AdipoGen Life Sciences
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetACE2
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-ACE2 (human), mAb (AC18F)
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AC18F
  • Concentration
    1 mg/ml
  • Gene ID59272
  • Target name
    ACE2
  • Target description
    angiotensin converting enzyme 2
  • Target synonyms
    ACEH; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; angiotensin-converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin-converting enzyme-related carboxypeptidase; metalloprotease MPROT15; peptidyl-dipeptidase A; truncated angiotensin converting enzyme 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9BYF1
  • Protein Name
    Angiotensin-converting enzyme 2
  • Scientific Description
    Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42% sequence identity with ACE, and its cytoplasmic and transmembrane domains show 48% homology to the protein collectrin that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19. - Monoclonal Antibody. Recognizes human ACE2. Isotype: Mouse IgG1kappa. Clone: AC18F. Applications: ELISA, FACS, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. ACE2 has direct effects on cardiac functions. It is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE2 converts angiotensin I to angiotensin 1-9. ACE2 was also identified as a functional receptor for SARS coronavirus.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACE2 using anti-ACE2 antibody (A00756-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE2 antigen affinity purified polyclonal antibody (Catalog # A00756-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE2 at approximately 120 kDa. The expected band size for ACE2 is at 93 kDa.
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