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Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Anti-ACHE Antibody

HPA027098
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetACHE
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACHE
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID43
  • Target name
    ACHE
  • Target description
    acetylcholinesterase (Cartwright blood group)
  • Target synonyms
    ACEE; acetylcholinesterase; acetylcholinesterase (Yt blood group); apoptosis-related acetylcholinesterase; ARACHE; N-ACHE; YT; Yt blood group
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22303
  • Protein Name
    Acetylcholinesterase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACHE using anti-ACHE antibody (PB9417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: PANC Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACHE antigen affinity purified polyclonal antibody (Catalog # PB9417) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACHE at approximately 68 kDa. The expected band size for ACHE is at 68 kDa.
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