Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001). Adiponectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001). Adiponectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Adiponectin/ADIPOQ Antibody Picoband(r)
PB9001-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetADIPOQ
Overview
- SupplierBoster Bio
- Product NameAnti-Adiponectin/ADIPOQ Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteWB: The detection limit for Adiponectin is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID9370
- Target nameADIPOQ
- Target descriptionadiponectin, C1Q and collagen domain containing
- Target synonyms30 kDa adipocyte complement-related protein; ACDC; ACRP30; adipocyte complement-related 30 kDa protein; adiponectin; adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPN; APM1; APM-1; GBP28; gelatin-binding protein 28
- HostRabbit
- IsotypeIgG
- Protein IDQ15848
- Protein NameAdiponectin
- Scientific DescriptionBoster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband® catalog # PB9001. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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