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Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.

Anti-AFF4 Antibody

HPA023690
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAFF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AFF4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27125
  • Target name
    AFF4
  • Target description
    ALF transcription elongation factor 4
  • Target synonyms
    AF5Q31, CHOPS, MCEF, AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, major CDK9 elongation factor-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHB7
  • Protein Name
    AF4/FMR2 family member 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of AFF4 using anti-AFF4 antibody (A03824). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFF4 antigen affinity purified polyclonal antibody (Catalog # A03824) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFF4 at approximately 150 kDa. The expected band size for AFF4 is at 127 kDa.
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