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Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.

Anti-AHSA1 Antibody

HPA000903
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAHSA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AHSA1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10598
  • Target name
    AHSA1
  • Target description
    activator of HSP90 ATPase activity 1
  • Target synonyms
    AHA1, C14orf3, hAha1, p38, activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95433
  • Protein Name
    Activator of 90 kDa heat shock protein ATPase homolog 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human MOLT4 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHA1/AHSA1 antigen affinity purified polyclonal antibody (Catalog # A05733-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHA1/AHSA1 at approximately 40 kDa. The expected band size for AHA1/AHSA1 is at 40 kDa.
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