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ProductsBack/AHA-1 antibody [N1C1]

AHA-1 antibody [N1C1]

GTX102312
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAHSA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AHA-1 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.98 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10598
  • Target name
    AHSA1
  • Target description
    activator of HSP90 ATPase activity 1
  • Target synonyms
    activator of 90 kDa heat shock protein ATPase homolog 1; AHA1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1; C14orf3; hAha1; p38
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95433
  • Protein Name
    Activator of 90 kDa heat shock protein ATPase homolog 1
  • Scientific Description
    Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • AHA1 regulates cell migration and invasion via the EMT pathway in colorectal adenocarcinomas. Kim D et al., 2021 Oct 7, Sci Rep
    Read more

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Figure 1. Western blot analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human MOLT4 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHA1/AHSA1 antigen affinity purified polyclonal antibody (Catalog # A05733-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHA1/AHSA1 at approximately 40 kDa. The expected band size for AHA1/AHSA1 is at 40 kDa.
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