
Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 10% SDS PAGE GTX102312 diluted at 1:1000
AHA-1 antibody [N1C1]
GTX102312
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetAHSA1
Overview
- SupplierGeneTex
- Product NameAHA-1 antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.98 mg/ml
- ConjugateUnconjugated
- Gene ID10598
- Target nameAHSA1
- Target descriptionactivator of HSP90 ATPase activity 1
- Target synonymsAHA1, C14orf3, hAha1, p38, activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1
- HostRabbit
- IsotypeIgG
- Protein IDO95433
- Protein NameActivator of 90 kDa heat shock protein ATPase homolog 1
- Scientific DescriptionCochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Kim D, Moon JW, Min DH, et al. AHA1 regulates cell migration and invasion via the EMT pathway in colorectal adenocarcinomas. Sci Rep. 2021,11(1):19946. doi: 10.1038/s41598-021-99375-wRead this paper
![AHA-1 antibody [N1C1] detects AHA-1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min AHA-1 antibody [N1C1] detects AHA-1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102312/GTX102312_40093_20150506_IHC_M_w_23060100_560.webp)


![Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102312/GTX102312_40093_20170601_WB_KO_watermark_w_23060100_821.webp)




