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anti-AKR1B10 antibody

ARG55510
Arigo Biolaboratories
ApplicationsWestern Blot
Product group Antibodies
TargetAKR1B10
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-AKR1B10 antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57016
  • Target name
    AKR1B10
  • Target description
    aldo-keto reductase family 1 member B10
  • Target synonyms
    AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI, aldo-keto reductase family 1 member B10, ARP, SI reductase, aldo-keto reductase family 1, member B10 (aldose reductase), aldo-keto reductase family 1, member B11 (aldose reductase-like), aldose reductase-like 1, aldose reductase-like peptide, aldose reductase-related protein, hARP, small intestine reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKR1B10 using anti-AKR1B10 antibody (A02976). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1B10 antigen affinity purified polyclonal antibody (Catalog # A02976) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1B10 at approximately 36 kDa. The expected band size for AKR1B10 is at 36 kDa.
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