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Anti-alpha-2 integrin inserted-domain [2E10]

Ab00777-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
TargetITGA2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-alpha-2 integrin inserted-domain [2E10]
  • Delivery Days Customer
    7
  • Application Supplier Note
    2E10 scFv can be used to detect alpha2 and alpha1 intergin I-domain in ELISA and FC studies.
  • Applications
    Flow Cytometry, ELISA
  • Applications Supplier
    ELISA; FC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2E10
  • Gene ID3673
  • Target name
    ITGA2
  • Target description
    integrin subunit alpha 2
  • Target synonyms
    alpha 2 subunit of VLA-2 receptor; BR; CD49 antigen-like family member B; CD49B; collagen receptor; GPIa; HPA-5; human platelet alloantigen system 5; integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2; VLAA2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17301
  • Protein Name
    Integrin alpha-2
  • Scientific Description
    This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG (scFv) format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    2E10 was generated by immunizing BALB/c mice with a DNA vaccine containing the human alpha2 I-domain cDNAsequence within pVAC2. The alpha2 I-domain is expressed as a fusion protein with fragment C of tetanus toxoid (FrC-TT). The scFv was then created using a V-gene phage display library derived from RNA from mice splenocytes and isolated following selection on GST-I after 3 rounds of panning.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 2/ITGA2 using anti-Integrin Alpha 2/ITGA2 antibody (A01933-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 2/ITGA2 antigen affinity purified polyclonal antibody (Catalog # A01933-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 2/ITGA2 at approximately 165 kDa. The expected band size for Integrin Alpha 2/ITGA2 is at 129 kDa.
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