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293T(Human) Cell Lysate at 30 ug probed with Integrin alpha 2/CD49b Polyclonal Antibody (bs-10132R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
293T(Human) Cell Lysate at 30 ug probed with Integrin alpha 2/CD49b Polyclonal Antibody (bs-10132R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
293T(Human) Cell Lysate at 30 ug probed with Integrin alpha 2/CD49b Polyclonal Antibody (bs-10132R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Integrin alpha 2 Polyclonal Antibody

BS-10132R
Bioss Antibodies
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetITGA2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Integrin alpha 2/CD49b Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3673
  • Target name
    ITGA2
  • Target description
    integrin subunit alpha 2
  • Target synonyms
    alpha 2 subunit of VLA-2 receptor; BR; CD49 antigen-like family member B; CD49B; collagen receptor; GPIa; HPA-5; human platelet alloantigen system 5; integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2; VLAA2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17301
  • Protein Name
    Integrin alpha-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Application of luteolin nanomicelles anti-glioma effect with improvement in vitro and in vivo. Zheng S et al., 2017 Sep 22, Oncotarget
    Read more

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Figure 1. Western blot analysis of Integrin Alpha 2/ITGA2 using anti-Integrin Alpha 2/ITGA2 antibody (A01933-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 2/ITGA2 antigen affinity purified polyclonal antibody (Catalog # A01933-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 2/ITGA2 at approximately 165 kDa. The expected band size for Integrin Alpha 2/ITGA2 is at 129 kDa.
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