More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-alpha-Fetoprotein Biotin

Anti-alpha-Fetoprotein Biotin

1B-259
EXBIO
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    EXBIO
  • Product Name
    Anti-alpha-Fetoprotein Biotin
  • Delivery Days Customer
    7
  • Application Supplier Note
    ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100). Western blotting: Recommended dilution: 1-2 microg/ml; non-reducing conditions. Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clone ID
    AFP-01
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Isotype
    IgG1
  • Scientific Description
    Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Abbexa
Alpha-Fetoprotein (AFP) AntibodyABX000589
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Alpha-fetoprotein (AFP) [AF5]AB00213-1.1-BT
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityHuman
TargetAFP
  • SizePrice
Anti-AFP Antibody
Product group Antibodies
Antibodies.com
Anti-AFP AntibodyA101653
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Biosynth
AFP antibody10-1003
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Human AFP Antibody130-10547
ApplicationsELISA
TargetAFP
  • SizePrice
Immunohistochemical staining of human liver shows moderate positivity in secretion.
Product group Antibodies
Atlas Antibodies
Anti-AFP AntibodyAMAB91610
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetAFP
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
AFP AntibodyLS-C831453
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Product group Antibodies
References
Bioss
AFP Monoclonal AntibodyBSM-1621M
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
TargetAFP
  • SizePrice
Figure 1. Western blot analysis of alpha 1 Fetoprotein/AFP using anti-alpha 1 Fetoprotein/AFP antibody (A00522-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 1 Fetoprotein/AFP antigen affinity purified polyclonal antibody (Catalog # A00522-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 1 Fetoprotein/AFP at approximately 69 kDa. The expected band size for alpha 1 Fetoprotein/AFP is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-alpha 1 Fetoprotein/AFP Antibody Picoband(r)A00522-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAFP
  • SizePrice
Western blot analysis of HepG2, diluted at 1:2,000.
Product group Antibodies
Cusabio
AFP Monoclonal AntibodyCSB-MA000233
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAFP
  • SizePrice