Anti-Alpha-synuclein [D10], Mouse IgG2b, Fc Silent(tm), kappa

Absolute Antibody

Anti-Alpha-synuclein [D10], Mouse IgG2b, Fc Silent(tm), kappa

9

This antibody binds human monomeric and high molecular weight forms of wild-type a-syn. Alpha-synuclein is a small 140 amino acid presynaptic neuronal protein, a major component of Lewy bodies and a member of the synuclein family. Misfolding, abnormal acc

This antibody is capable of binding recombinant human alpha-synuclein in an ELISA. The intracellular binding of scFv D10 antibody to alpha-syn was studied by cotransfection of COS-7 cells with a D10-HA-NLS plasmid (hemagglutinin (HA) tag to detect scFv; nuclear localization signal (NLS) for retargeting to nucleus) and an alpha-syn plasmid. Immunocytochemical staining of HEK 293/alpha-syn/D10 cells reveals that the D10 intrabody has a predominantly cytoplasmic distribution and does not induce visible alpha-syn aggregate/inclusion formation. Transfection of D10 scFv gene into an HEK 293 cell line that overexpresses wild-type alpha-syn, showed that the D10 intrabody stabilizes detergent-soluble monomeric alpha-syn and inhibits the formation of detergent-insoluble high-molecular-weight alpha-syn species. It was seen that this antibody also ameliorated decreased cell adhesion characterized by alpha-syn-overexpressing cells (PMID: 15564134). This antibody is capable of detecting total alpha-syn in a western blot (PMID: 26287258).

Western Blot, ELISA, ImmunoCytoChemistry

Research Use Only

Monoclonal

D10

SNCA

synuclein alpha

alpha-synuclein; I+/--synuclein; NACP; non A-beta component of AD amyloid; PARK1; PARK4; PD1; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); truncated alpha synuclein

Mouse

IgG2b

Alpha-synuclein

This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse scFv format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203