Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX112799/GTX112799_42606_20170531_IHC-Fr_M_w_23060500_627.webp "alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112799/GTX112799_42606_42886_IHC-Fr_M_w_23060500_347.webp "alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.")
![alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX112799/GTX112799_42606_20170719_IFA_R_w_23060500_399.webp "alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Synuclein antibody
GTX112799
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNCA
Overview
- SupplierGeneTex
- Product Namealpha Synuclein antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.31 mg/ml
- ConjugateUnconjugated
- Gene ID6622
- Target nameSNCA
- Target descriptionsynuclein alpha
- Target synonymsNACP, PARK1, PARK4, PD1, alpha-synuclein, I+/--synuclein, non A-beta component of AD amyloid, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor)
- HostRabbit
- IsotypeIgG
- Protein IDP37840
- Protein NameAlpha-synuclein
- Scientific DescriptionAlpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimers disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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