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Anti-AML1-ETO Polyclonal Antibody

Anti-AML1-ETO Polyclonal Antibody

25208
BPS Bioscience
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
Product group Antibodies
TargetRUNX1
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    Anti-AML1-ETO Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
  • Applications Supplier
    ChIP Chromatin ImmunoPrecipitation; ELISA; Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID861
  • Target name
    RUNX1
  • Target description
    RUNX family transcription factor 1
  • Target synonyms
    AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha, runt-related transcription factor 1, AML1-ETO fusion, AML1-ETO fusion protein, AML1-EVI-1 fusion protein, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, acute myeloid leukemia 1 protein, core-binding factor, runt domain, alpha subunit 2, mutant RUNX1, oncogene AML-1, polyomavirus enhancer-binding protein 2 alpha B subunit, runt related transcription factor 1
  • Protein IDQ01196
  • Protein Name
    Runt-related transcription factor 1
  • Scientific Description
    Polyclonal antibody raised in rabbit against the AML1-ETO fusion protein using a KLH-conjugated synthetic peptide.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RUNX1 using anti-RUNX1 antibody (PB9157). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1 antigen affinity purified polyclonal antibody (Catalog # PB9157) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX1 at approximately 55KD. The expected band size for RUNX1 is at 49KD.
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