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Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic positivity in glandular cells.

Anti-ANAPC1 Antibody

HPA036329
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetANAPC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ANAPC1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64682
  • Target name
    ANAPC1
  • Target description
    anaphase promoting complex subunit 1
  • Target synonyms
    APC1, MCPR, TSG24, anaphase-promoting complex subunit 1, anaphase-promoting complex 1 (meiotic checkpoint regulator), cyclosome subunit 1, mitotic checkpoint regulator, testis-specific gene 24 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1A4
  • Protein Name
    Anaphase-promoting complex subunit 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of APC1/ANAPC1 using anti-APC1/ANAPC1 antibody (A03471-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APC1/ANAPC1 antigen affinity purified polyclonal antibody (Catalog # A03471-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APC1/ANAPC1 at approximately 240 kDa. The expected band size for APC1/ANAPC1 is at 217 kDa.
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