More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/APC1 antibody
IHC-P analysis of human brain tissue using GTX31801 APC1 antibody. Working concentration : 5 microg/ml
IHC-P analysis of human brain tissue using GTX31801 APC1 antibody. Working concentration : 5 microg/ml
IHC-P analysis of human brain tissue using GTX31801 APC1 antibody. Working concentration : 5 microg/ml

APC1 antibody

GTX31801
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetANAPC1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    APC1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64682
  • Target name
    ANAPC1
  • Target description
    anaphase promoting complex subunit 1
  • Target synonyms
    anaphase-promoting complex 1 (meiotic checkpoint regulator); anaphase-promoting complex subunit 1; APC1; cyclosome subunit 1; MCPR; mitotic checkpoint regulator; testis-specific gene 24 protein; TSG24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1A4
  • Protein Name
    Anaphase-promoting complex subunit 1
  • Scientific Description
    This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of various cells using APC1 Polyclonal Antibody
Product group Antibodies
Cusabio
ANAPC1 AntibodyCSB-PA000898
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
TargetANAPC1
  • SizePrice
Western blot using GeneTex Affinity Purified anti-APC1 pS355 antibody (GTX10923) shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (GTX27090) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
APC1 (phospho Ser355) antibodyGTX10923
ApplicationsImmunoPrecipitation, Western Blot, ELISA
TargetANAPC1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ANAPC1 / APC1 AntibodyLS-C332600
ApplicationsWestern Blot
TargetANAPC1
  • SizePrice
Product group Antibodies
Bioss Antibodies
phospho-APC1 (Ser688) Polyclonal AntibodyBS-12482R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetANAPC1
  • SizePrice
Anti-ANAPC1 Antibody
Product group Antibodies
Antibodies.com
Anti-ANAPC1 AntibodyA29366
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-APC1 Antibody, Affinity PurifiedA301-653A
ApplicationsImmunoPrecipitation, Western Blot
TargetANAPC1
  • SizePrice
Product group Antibodies
MedChem Express
ANAPC1 Antibody (YA4486, Mouse)HY-P84789
TargetANAPC1
  • SizePrice
Figure 1. Western blot analysis of APC1/ANAPC1 using anti-APC1/ANAPC1 antibody (A03471-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APC1/ANAPC1 antigen affinity purified polyclonal antibody (Catalog # A03471-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APC1/ANAPC1 at approximately 240 kDa. The expected band size for APC1/ANAPC1 is at 217 kDa.
Product group Antibodies
Boster Bio
Anti-APC1/ANAPC1 Antibody Picoband(r)A03471-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetANAPC1
  • SizePrice
Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-ANAPC1 AntibodyHPA036329
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetANAPC1
  • SizePrice