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Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Anti-ANGPTL3 Antibody

HPA038097
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetANGPTL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ANGPTL3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27329
  • Target name
    ANGPTL3
  • Target description
    angiopoietin like 3
  • Target synonyms
    ANG-5, ANGPT5, ANL3, FHBL2, angiopoietin-related protein 3, angiopoietin 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5C1
  • Protein Name
    Angiopoietin-related protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ANGPTL3 using anti-ANGPTL3 antibody (A02929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPTL3 antigen affinity purified polyclonal antibody (Catalog # A02929) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPTL3 at approximately 54KD. The expected band size for ANGPTL3 is at 54KD.
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