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Anti-Ankyrin-R [N388A/10]

Ab02181-2.0
Absolute Antibody
ApplicationsOther Application
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetANK1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Ankyrin-R [N388A/10]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The IgG2b version of this antibody was used in a proximity ligation assay on red blood cells (Genetet et al, 2017; PMID:28387307).
  • Applications
    Other Application
  • Applications Supplier
    Proximity ligation assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    N388A/10
  • Gene ID286
  • Target name
    ANK1
  • Target description
    ankyrin 1
  • Target synonyms
    ANK, SPH1, SPH2, ankyrin-1, ankyrin-1, ANK-1, ankyrin 1, erythrocytic, ankyrin-R, erythrocyte ankyrin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP16157
  • Protein Name
    Ankyrin-1
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    human, mouse, rat
  • Reactivity Supplier Note
    This antibody was raised by immunising BALB/c mice with the ankyrinR null antigen.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ankyrin Erythroid/ANK/ANK1 using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ankyrin Erythroid/ANK/ANK1 antigen affinity purified polyclonal antibody (Catalog # A02716-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ankyrin Erythroid/ANK/ANK1 at approximately 250 kDa. The expected band size for Ankyrin Erythroid/ANK/ANK1 is at 206 kDa.
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