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Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Anti-APBA3 Antibody

HPA045577
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAPBA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-APBA3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9546
  • Target name
    APBA3
  • Target description
    amyloid beta precursor protein binding family A member 3
  • Target synonyms
    MGC:15815, X11L2, mint3, amyloid-beta A4 precursor protein-binding family A member 3, X11-like 2 protein, adapter protein X11gamma, amyloid beta (A4) precursor protein-binding, family A, member 3 (X11-like 2), mint-3, neuron-specific X11L2 protein, neuronal munc18-1-interacting protein 3, phosphotyrosine-binding/-interacting domain (PTB)-bearing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO96018
  • Protein Name
    Amyloid-beta A4 precursor protein-binding family A member 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APBA3 using anti-APBA3 antibody (A07396-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBA3 antigen affinity purified polyclonal antibody (Catalog # A07396-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBA3 at approximately 69 kDa. The expected band size for APBA3 is at 61 kDa.
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