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APBA3 Antibody

CSB-PA080020
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAPBA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    APBA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9546
  • Target name
    APBA3
  • Target description
    amyloid beta precursor protein binding family A member 3
  • Target synonyms
    MGC:15815, X11L2, mint3, amyloid-beta A4 precursor protein-binding family A member 3, X11-like 2 protein, adapter protein X11gamma, amyloid beta (A4) precursor protein-binding, family A, member 3 (X11-like 2), mint-3, neuron-specific X11L2 protein, neuronal munc18-1-interacting protein 3, phosphotyrosine-binding/-interacting domain (PTB)-bearing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO96018
  • Protein Name
    Amyloid-beta A4 precursor protein-binding family A member 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APBA3 using anti-APBA3 antibody (A07396-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBA3 antigen affinity purified polyclonal antibody (Catalog # A07396-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBA3 at approximately 69 kDa. The expected band size for APBA3 is at 61 kDa.
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