Figure 2. IHC analysis of APE1 using anti-APE1 antibody (M00627). APE1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml mouse anti-APE1 Antibody (M00627) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Figure 2. IHC analysis of APE1 using anti-APE1 antibody (M00627). APE1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml mouse anti-APE1 Antibody (M00627) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Anti-APE1 APEX1 Antibody Picoband(r) (monoclonal, 5C11)
M00627
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAPEX1
Overview
- SupplierBoster Bio
- Product NameAnti-APE1 Antibody (monoclonal, 5C11)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5C11
- Concentration500 ug/ml
- Gene ID328
- Target nameAPEX1
- Target descriptionapurinic/apyrimidinic endodeoxyribonuclease 1
- Target synonymsAP endonuclease class I; AP lyase; APE; APE1; APEN; APEX; APEX nuclease (multifunctional DNA repair enzyme) 1; apurinic/apyrimidinic (abasic) endonuclease; apurinic-apyrimidinic endonuclease 1; APX; deoxyribonuclease (apurinic or apyrimidinic); DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; HAP1; protein REF-1; redox factor-1; REF1
- HostMouse
- IsotypeIgG2b
- Protein IDP27695
- Protein NameDNA-(apurinic or apyrimidinic site) lyase
- Scientific DescriptionBoster Bio Anti-APE1 APEX1 Antibody Picoband® (monoclonal, 5C11) catalog # M00627. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203