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Immunohistochemical staining of human duodenum shows moderate positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate positivity in glandular cells.

Anti-ARHGEF5 Antibody

HPA044188
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARHGEF5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARHGEF5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7984
  • Target name
    ARHGEF5
  • Target description
    Rho guanine nucleotide exchange factor 5
  • Target synonyms
    GEF5, P60, TIM, TIM1, rho guanine nucleotide exchange factor 5, Rho guanine nucleotide exchange factor (GEF) 5, ephexin-3, ephexin3, guanine nucleotide regulatory protein TIM, oncogene TIM, p60 TIM, transforming immortalized mammary oncogene
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12774
  • Protein Name
    Rho guanine nucleotide exchange factor 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ARHGEF5 using anti-ARHGEF5 antibody (A01487-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF5 antigen affinity purified polyclonal antibody (Catalog # A01487-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF5 at approximately 250 kDa. The expected band size for ARHGEF5 is at 177 kDa.
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