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Immunohistochemical staining of human colorectal cancer shows weak to moderate nuclear positivity in tumor cells.
Immunohistochemical staining of human colorectal cancer shows weak to moderate nuclear positivity in tumor cells.
Immunohistochemical staining of human colorectal cancer shows weak to moderate nuclear positivity in tumor cells.

Anti-ARID1A Antibody

HPA005456
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetARID1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARID1A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8289
  • Target name
    ARID1A
  • Target description
    AT-rich interaction domain 1A
  • Target synonyms
    ARID domain-containing protein 1A; AT rich interactive domain 1A (SWI-like); AT-rich interactive domain-containing protein 1A; B120; BAF250; BAF250a; BM029; brain protein 120; BRG1-associated factor 250a; C1orf4; chromatin remodeling factor p250; CSS2; ELD; hELD; hOSA1; MRD14; osa homolog 1; OSA1; OSA1 nuclear protein; P270; SMARCF1; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; SWI-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14497
  • Protein Name
    AT-rich interactive domain-containing protein 1A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARID1A using anti-ARID1A antibody (A00247-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID1A antigen affinity purified polyclonal antibody (Catalog # A00247-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID1A at approximately 250-270 kDa. The expected band size for ARID1A is at 242 kDa.
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