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Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.

Anti-ARL1 Antibody

HPA062656
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetARL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARL1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID400
  • Target name
    ARL1
  • Target description
    ARF like GTPase 1
  • Target synonyms
    ARFL1, ADP-ribosylation factor-like protein 1, ADP ribosylation factor like GTPase 1, ADP-ribosylation factor-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40616
  • Protein Name
    ADP-ribosylation factor-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of ARL1 using anti-ARL1 antibody (A03733-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL1 antigen affinity purified polyclonal antibody (Catalog # A03733-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL1 at approximately 20 kDa. The expected band size for ARL1 is at 20 kDa.
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