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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ARL1 antibody at 5.1microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 19 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ARL1 antibody at 5.1microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 19 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ARL1 antibody at 5.1microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 19 kDa Observed band size: 21 kDa

ARL1 Antibody

CSB-PA002070LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID400
  • Target name
    ARL1
  • Target description
    ARF like GTPase 1
  • Target synonyms
    ARFL1, ADP-ribosylation factor-like protein 1, ADP ribosylation factor like GTPase 1, ADP-ribosylation factor-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40616
  • Protein Name
    ADP-ribosylation factor-like protein 1
  • Scientific Description
    GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of ARL1 using anti-ARL1 antibody (A03733-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL1 antigen affinity purified polyclonal antibody (Catalog # A03733-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL1 at approximately 20 kDa. The expected band size for ARL1 is at 20 kDa.
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