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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.

Anti-ARL3 Antibody

HPA036292
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetARL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARL3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID403
  • Target name
    ARL3
  • Target description
    ARF like GTPase 3
  • Target synonyms
    ARFL3, JBTS35, RP83, ADP-ribosylation factor-like protein 3, ADP ribosylation factor like GTPase 3, ADP-ribosylation factor-like 3, ARF-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36405
  • Protein Name
    ADP-ribosylation factor-like protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARL3 using anti-ARL3 antibody (A04080-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL3 antigen affinity purified polyclonal antibody (Catalog # A04080-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL3 at approximately 23 kDa. The expected band size for ARL3 is at 20 kDa.
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