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Gel: 15%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Mouse brain tissue, 231 cells, A375 cells, Primary antibody: CSB-PA286880(ARL3 Antibody) at dilution 1/433, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 15%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Mouse brain tissue, 231 cells, A375 cells, Primary antibody: CSB-PA286880(ARL3 Antibody) at dilution 1/433, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 15%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Mouse brain tissue, 231 cells, A375 cells, Primary antibody: CSB-PA286880(ARL3 Antibody) at dilution 1/433, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes

ARL3 Antibody

CSB-PA286880
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARL3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARL3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID403
  • Target name
    ARL3
  • Target description
    ADP ribosylation factor like GTPase 3
  • Target synonyms
    ADP-ribosylation factor-like 3; ADP-ribosylation factor-like protein 3; ARFL3; ARF-like 3; JBTS35; RP83
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36405
  • Protein Name
    ADP-ribosylation factor-like protein 3
  • Scientific Description
    ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARL3 using anti-ARL3 antibody (A04080-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL3 antigen affinity purified polyclonal antibody (Catalog # A04080-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL3 at approximately 23 kDa. The expected band size for ARL3 is at 20 kDa.
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