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Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.

Anti-ARSE Antibody

HPA070651
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARSL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARSE Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID415
  • Target name
    ARSL
  • Target description
    arylsulfatase L
  • Target synonyms
    ARSE, ASE, CDPX, CDPX1, CDPXR, arylsulfatase L, arylsulfatase E (chondrodysplasia punctata 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51690
  • Protein Name
    Arylsulfatase L
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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IHC image of CSB-PA002146LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of ARSE/ASE/ARSL using anti-ARSE/ASE/ARSL antibody (A10294-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: monkey Cos-7 whole cell lysates, Lane 3: human HK-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARSE/ASE/ARSL antigen affinity purified polyclonal antibody (Catalog # A10294-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARSE/ASE/ARSL at approximately 66KD. The expected band size for ARSE/ASE/ARSL is at 66KD.
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