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ProductsBack/ARSE antibody [C2C3], C-term

ARSE antibody [C2C3], C-term

GTX101335
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetARSL
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Overview

  • Supplier
    GeneTex
  • Product Name
    ARSE antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID415
  • Target name
    ARSL
  • Target description
    arylsulfatase L
  • Target synonyms
    ARSE, ASE, CDPX, CDPX1, CDPXR, arylsulfatase L, arylsulfatase E (chondrodysplasia punctata 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51690
  • Protein Name
    Arylsulfatase L
  • Scientific Description
    Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development, has been linked to mutations in this gene. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARSE/ASE/ARSL using anti-ARSE/ASE/ARSL antibody (A10294-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: monkey Cos-7 whole cell lysates, Lane 3: human HK-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARSE/ASE/ARSL antigen affinity purified polyclonal antibody (Catalog # A10294-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARSE/ASE/ARSL at approximately 66KD. The expected band size for ARSE/ASE/ARSL is at 66KD.
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