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Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.

Anti-ASAH1 Antibody

HPA005468
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetASAH1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ASAH1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID427
  • Target name
    ASAH1
  • Target description
    N-acylsphingosine amidohydrolase 1
  • Target synonyms
    AC; ACDase; acid CDase; acid ceramidase; acylsphingosine deacylase; ASAH; N-acylethanolamine hydrolase ASAH1; N-acylsphingosine amidohydrolase (acid ceramidase) 1; PHP; PHP32; putative 32 kDa heart protein; SMAPME
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13510
  • Protein Name
    Acid ceramidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASAH1 antigen affinity purified polyclonal antibody (Catalog # A02055-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASAH1 at approximately 45KD. The expected band size for ASAH1 is at 45KD.
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