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Anti-ASC/TMS1/Pycard Antibody

ORB2588500
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetPycard
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-ASC/TMS1/Pycard Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.25-0.5 microg/ml, Mouse Immunocytochemistry/Immunofluorescence, 5 microg/ml, Mouse Flow Cytometry (Fixed), 1-3 microg/1x1x106 cells, Mouse ELISA, 0.1-0.5 microg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 microg/ml
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    ELISA, FC, ICC, IF, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Biotin
  • Gene ID66824
  • Target name
    Pycard
  • Target description
    PYD and CARD domain containing
  • Target synonyms
    9130417A21Rik; A; apoptosis-associated speck-like protein containing a CARD; Asc; CARD5; masc; PYD and CARD domain-containing protein; TMS-; TMS-1; TNS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9EPB4
  • Protein Name
    Apoptosis-associated speck-like protein containing a CARD
  • Scientific Description
    Anti-ASC/TMS1/Pycard Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMS1/ASC/PYCARD using anti-TMS1/ASC/PYCARD antibody (A00362-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMS1/ASC/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMS1/ASC/PYCARD at approximately 22-25 kDa. The expected band size for TMS1/ASC/PYCARD is at 21 kDa.
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Anti-TMS1/ASC/Pycard Antibody Picoband(r)A00362-4-CARRIER-FREE
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TargetPycard
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