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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.

Anti-ASF1B Antibody

HPA054036
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetASF1B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ASF1B
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55723
  • Target name
    ASF1B
  • Target description
    anti-silencing function 1B histone chaperone
  • Target synonyms
    anti-silencing function protein 1 homolog B; ASF1 anti-silencing function 1 homolog B; CCG1-interacting factor A-II; CIA-II; hAsf1; hAsf1b; hCIA-II; histone chaperone ASF1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVP2
  • Protein Name
    Histone chaperone ASF1B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Immunohistochemistry analysis in human testis and cerebellum tissues using Anti-ASF1B antibody. Corresponding ASF1B RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of ASF1B using anti-ASF1B antibody (A05211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASF1B antigen affinity purified polyclonal antibody (Catalog # A05211-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASF1B at approximately 18 kDa. The expected band size for ASF1B is at 18 kDa.
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