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ASF1B Antibody

CSB-PA030225
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetASF1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    ASF1B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55723
  • Target name
    ASF1B
  • Target description
    anti-silencing function 1B histone chaperone
  • Target synonyms
    CIA-II, histone chaperone ASF1B, ASF1 anti-silencing function 1 homolog B, CCG1-interacting factor A-II, anti-silencing function protein 1 homolog B, hAsf1, hAsf1b, hCIA-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVP2
  • Protein Name
    Histone chaperone ASF1B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ASF1B using anti-ASF1B antibody (A05211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASF1B antigen affinity purified polyclonal antibody (Catalog # A05211-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASF1B at approximately 18 kDa. The expected band size for ASF1B is at 18 kDa.
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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
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