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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.

Anti-ASIC1 Antibody

HPA058870
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetASIC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ASIC1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID41
  • Target name
    ASIC1
  • Target description
    acid sensing ion channel subunit 1
  • Target synonyms
    ACCN2, ASIC, BNaC2, acid-sensing ion channel 1, Cation channel, amiloride-sensitive, neuronal, 2, acid sensing (proton gated) ion channel 1, acid-sensing ion channel 1a protein, amiloride-sensitive cation channel 2, neuronal, brain sodium channel 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78348
  • Protein Name
    Acid-sensing ion channel 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ASIC1 using anti-ASIC1 antibody (A02332-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC1 antigen affinity purified polyclonal antibody (Catalog # A02332-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC1 at approximately 60KD. The expected band size for ASIC1 is at 60KD.
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