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Anti-ATG12 (N-term) Antibody

102-20870
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetATG12
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-ATG12 (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB2793
  • Gene ID9140
  • Target name
    ATG12
  • Target description
    autophagy related 12
  • Target synonyms
    APG12; Apg12 (autophagy, yeast) homolog; APG12 autophagy 12-like; APG12L; ATG12 autophagy related 12 homolog; FBR93; HAPG12; ubiquitin-like protein ATG12
  • Host
    Rabbit
  • Protein IDO94817
  • Protein Name
    Ubiquitin-like protein ATG12
  • Scientific Description
    Rabbit Anti-ATG12 (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG12 using anti-ATG12 antibody (A00820-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human COLO 320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG12 antigen affinity purified polyclonal antibody (Catalog # A00820-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG12 at approximately 48 kDa. The expected band size for ATG12 is at 15 kDa.
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