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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATG12 antibody

GTX124181
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATG12
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATG12 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.43 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9140
  • Target name
    ATG12
  • Target description
    autophagy related 12
  • Target synonyms
    APG12, APG12L, FBR93, HAPG12, ubiquitin-like protein ATG12, APG12 autophagy 12-like, ATG12 autophagy related 12 homolog, Apg12 (autophagy, yeast) homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94817
  • Protein Name
    Ubiquitin-like protein ATG12
  • Scientific Description
    Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Okada T, Ogura T. Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells. Int J Mol Sci. 2021,22(4). doi: 10.3390/ijms22041834
    Read this paper
  • Ho KH, Lee YT, Chen PH, et al. Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling. Neurotherapeutics. 2021,18(2):1371-1392. doi: 10.1007/s13311-020-00961-z
    Read this paper
  • Lin TY, Chan HH, Chen SH, et al. BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells. Autophagy. 2020,16(7):1296-1313. doi: 10.1080/15548627.2019.1671643
    Read this paper
  • Chen JC, Hsieh YY, Lo HL, et al. In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells. Biomolecules. 2019,9(9). doi: 10.3390/biom9090467
    Read this paper
  • Hsu HL, Liao PL, Cheng YW, et al. Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells. Int J Mol Sci. 2019,20(1). doi: 10.3390/ijms20010157
    Read this paper
  • Lai CH, Huang JC, Cheng HH, et al. Helicobacter pylori cholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages. Cell Microbiol. 2018,20(12):e12947. doi: 10.1111/cmi.12947
    Read this paper
  • Liu YH, Weng YP, Tsai HY, et al. Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells. Phytomedicine. 2018,46:184-192. doi: 10.1016/j.phymed.2018.03.037
    Read this paper
  • Koh EH, Chernis N, Saha PK, et al. miR-30a Remodels Subcutaneous Adipose Tissue Inflammation to Improve Insulin Sensitivity in Obesity. Diabetes. 2018,67(12):2541-2553. doi: 10.2337/db17-1378
    Read this paper
  • Kuo SM, Chen CJ, Chang SC, et al. Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy. mBio. 2017,8(3). doi: 10.1128/mBio.00481-17
    Read this paper
  • Yen JH, Wu PS, Chen SF, et al. Fisetin Protects PC12 Cells from Tunicamycin-Mediated Cell Death via Reactive Oxygen Species Scavenging and Modulation of Nrf2-Driven Gene Expression, SIRT1 and MAPK Signaling in PC12 Cells. Int J Mol Sci. 2017,18(4). doi: 10.3390/ijms18040852
    Read this paper

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