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Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using Anti-ATL1 antibody. Corresponding ATL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using Anti-ATL1 antibody. Corresponding ATL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using Anti-ATL1 antibody. Corresponding ATL1 RNA-seq data are presented for the same tissues.

Anti-ATL1 Antibody

HPA027550
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetATL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ATL1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51062
  • Target name
    ATL1
  • Target description
    atlastin GTPase 1
  • Target synonyms
    AD-FSP, ATL-1, FSP1, GBP3, HSN1D, SPG3, SPG3A, atlastin1, atlastin-1, GBP-3, GTP-binding protein 3, brain-specific GTP-binding protein, guanine nucleotide-binding protein 3, guanylate-binding protein 3, hGBP3, spastic paraplegia 3 protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WXF7
  • Protein Name
    Atlastin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA855500LA01HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPG3A/ATL1 antigen affinity purified polyclonal antibody (Catalog # A01994-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPG3A/ATL1 at approximately 64 kDa. The expected band size for SPG3A/ATL1 is at 64 kDa.
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