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ATL1 Antibody

LS-C333967
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetATL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATL1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 63kDa, while the observed MW by Western blot was 64kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 63kDa, while the observed MW by Western blot was 64kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.973 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51062
  • Target name
    ATL1
  • Target description
    atlastin GTPase 1
  • Target synonyms
    AD-FSP, ATL-1, FSP1, GBP3, HSN1D, SPG3, SPG3A, atlastin1, atlastin-1, GBP-3, GTP-binding protein 3, brain-specific GTP-binding protein, guanine nucleotide-binding protein 3, guanylate-binding protein 3, hGBP3, spastic paraplegia 3 protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPG3A/ATL1 antigen affinity purified polyclonal antibody (Catalog # A01994-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPG3A/ATL1 at approximately 64 kDa. The expected band size for SPG3A/ATL1 is at 64 kDa.
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