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ProductsBack/Anti-ATP1A2 Rabbit Monoclonal Antibody
Western blot analysis of ATP1A2 expression in Human fetal heart lysate.
Western blot analysis of ATP1A2 expression in Human fetal heart lysate.
Western blot analysis of ATP1A2 expression in Human fetal heart lysate.

Anti-ATP1A2 Rabbit Monoclonal Antibody

M02064
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATP1A2
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-ATP1A2 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    25A95
  • Gene ID477
  • Target name
    ATP1A2
  • Target description
    ATPase Na+/K+ transporting subunit alpha 2
  • Target synonyms
    DEE98, FARIMPD, FHM2, MHP2, sodium/potassium-transporting ATPase subunit alpha-2, ATPase Na+/K+ transporting alpha 2 polypeptide, Na(+)/K(+) ATPase alpha-2 subunit, Na+/K+ ATPase, alpha-A(+) catalytic polypeptide, Na+/K+ ATPase, alpha-B polypeptide, sodium pump subunit alpha-2, sodium-potassium ATPase catalytic subunit alpha-2, sodium/potassium-transporting ATPase alpha-2 chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50993
  • Protein Name
    Sodium/potassium-transporting ATPase subunit alpha-2
  • Scientific Description
    Boster Bio Anti-ATP1A2 Rabbit Monoclonal Antibody catalog # M02064. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ATP1A2 using anti-ATP1A2 antibody (A02064-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,(100°C boiled for 5 minutes) Lane 2: rat brain tissue lysates,(37°C incubated for 10 minutes) Lane 3: mouse brain tissue lysates,(100°C boiled for 5 minutes) Lane 4: mouse brain tissue lysates.(37°C incubated for 10 minutes) After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A2 antigen affinity purified polyclonal antibody (Catalog # A02064-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A2 at approximately 112 kDa. The expected band size for ATP1A2 is at 112 kDa.
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