Figure 1. Western blot analysis of ATP1A3 using anti-ATP1A3 antibody (A02278-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A3 antigen affinity purified polyclonal antibody (Catalog # A02278-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A3 at approximately 90 kDa. The expected band size for ATP1A3 is at 112 kDa.
Figure 1. Western blot analysis of ATP1A3 using anti-ATP1A3 antibody (A02278-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A3 antigen affinity purified polyclonal antibody (Catalog # A02278-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A3 at approximately 90 kDa. The expected band size for ATP1A3 is at 112 kDa.
Anti-ATP1A3 Antibody Picoband(r)
A02278-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATP1A3
Overview
- SupplierBoster Bio
- Product NameAnti-ATP1A3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID478
- Target nameATP1A3
- Target descriptionATPase Na+/K+ transporting subunit alpha 3
- Target synonymsAHC2; ATP1A1; ATPase, Na+/K+ transporting, alpha 3 polypeptide; CAPOS; DYT12; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; Na+, K+ activated adenosine triphosphatase alpha subunit; Na+/K+ ATPase 3; RDP; sodium pump subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain; sodium/potassium-transporting ATPase subunit alpha-3; sodium-potassium ATPase catalytic subunit alpha-3; sodium-potassium-ATPase, alpha 3 polypeptide
- HostRabbit
- Protein IDP13637
- Protein NameSodium/potassium-transporting ATPase subunit alpha-3
- Scientific DescriptionBoster Bio Anti-ATP1A3 Antibody Picoband® catalog # A02278-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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